Putting It All Together The New ChemiDoc MP Imaging System This new coating also provides resistance to solarization, in which a material exhibits changes in color or emissivity after exposure to ultraviolet light, but can also progressively degrade the physical or mechanical properties of many materials, especially polymers, after prolonged exposure. This results in lower overall sensitivity of the system.įor the ChemiDoc MP System, we selected materials and coatings for the interior surfaces specifically to reduce fluorescence and thus eliminate this source of background. If any materials show autofluorescence and emit light within the bandpass of the emission filters, then background levels of the image will increase. With fluorescent western blots, the blot and some of the interior of the instrument is subjected to intense excitation light. Many materials will fluoresce when exposed to high intensity light, termed autofluorescence. Would have caused increased background in the images. Time caused the uncoated part of this component to fluoresce, which Our R&D team has done as much of that work for you as possible, by examining all possible sources of error then refining and optimizing the components, optics, electronics, and software to yield consistent results.Īutofluorescenece Suppression: Solarization due to UV exposure over For example, will you get the same results when a blot is placed on different areas of the platen? Imaging is an experiment too, and needs experimental controls in place for accuracy. The end user generally assumes that an instrument just works the way it’s supposed to.īut that can be a risky assumption, and doing good science means accounting for all sources of error. Researchers focused on their biological questions shouldn't also need to be experts in imaging. While obvious imaging artifacts are easily noticed, faint or missing bands, poor sensitivity, inconsistent results, inaccurate quantitation, and other issues may be mistaken as simply the way an instrument functions. Limitations in an imaging system may not be readily apparent to the user. See what differentiates a merely good imager from a truly great imaging system. 22.5 x 18.Learn how Bio-Rad imaging systems are custom- designed for peak performance from the leaders of our Imaging R&D team.Multiple image analysis options: perform densitometry, quantitation, and normalization directly using the on-instrument software, or for more in-depth analysis use iBright Analysis Software (available in both desktop and cloud-based versions).Flexible connectivity: export captured images via ethernet connection, Wi-Fi (with optional accessory), USB, or directly to Connect cloud-based platform.Green LED-based transilluminator: effectively excite popular DNA dyes such as ethidium bromide and Invitrogen SYBR Green dyes with an alternative to UV-based transilluminators.22.5 x 18.0 cm field of view: large field of view for high-throughput imaging (image up to four mini or two midi blots at a time).Advanced automated features: automatic sample rotation, automatic zoom, and automatic focus help streamline image capture.Simple interface: clear layout of functions and features combined with a 12.1-inch capacitive touchscreen for a smooth imaging experience.Smart Exposure technology: provides rapid determination of optimal exposure time to help minimize the need to repeat exposures to acquire the desired signal.Five fluorescence channels: multiplex and capture up to four proteins in a single blot for more meaningful and representative experiments (FL1500 model only).9.1 MP cooled CCD camera: high sensitivity and dynamic range to help enable the detection of subtle differences in samples.Feature overview of the iBright Imaging Systems:
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